Published August 17, 2004
by Humana Press .
Written in English
Methods in Molecular Biology
|The Physical Object|
|Number of Pages||496|
This book will provide a newcomer to NMR with the practical guidance in order to carry out successful experiments with proteins and to analyze the resulting spectra. Those who are familiar with the chemical applications of NMR will also find is useful in understanding the special requirements of protien : Hardcover. Protein NMR: Modern Techniques and Biomedical Applications (Biological Magnetic Resonance Book 32) - Kindle edition by Lawrence Berliner. Download it once and read it on your Kindle device, PC, phones or tablets. Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR. In Protein NMR Techniques leading experts demonstrate how to successfully apply the fundamentals of NMR to the study of proteins. They cover not only assignment and structure determination techniques, but also the practical aspects of experimental set-up, protein production, data .
This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies Author: Alexander Shekhtman. This book covers new techniques in protein NMR, from basic principles to state-of-the-art research. It covers a spectrum of topics ranging from a “toolbox” for how sequence-specific resonance assignments can be obtained using a suite of 2D and 3D NMR experiments . NMR is also a very powerful method for the study of interactions of protein with other molecules, whether small molecules (including drugs), nuclear acids or other proteins. This up-to-date volume covers NMR techiniques and their application to proteins, with a focus on practical details. Key words: High yield protein production, Bacterial expression, Isotopic labeling, NMR 1. Introduction To perform NMR structural studies of proteins, we have to produce proteins that are isotopically labeled with 13C and 15N for small proteins (proteins .
State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules. From reviews of the first edition. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules. "Protein NMR Spectroscopy: Principles and Practice covers a huge range of topics related to NMR.A fine two-semester course could be built on this very thorough book. Any student currently using NMR could benefit from the Cavanagh et al. text, which provides an in-depth explanation that is suitable as a resource for even a knowledgeable spectroscopist.". Protein NMR Techniques offers a practical blend of in-depth theory and practice, as well as worked-out examples of the use of NMR in studying the properties of proteins. Its focused treatment of proteins make it an indispensable study guide for chemists and biochemists, including both those with only a basic knowledge of NMR, as well as more experienced investigators.